BioFab RBS

apFAB563

This part belongs to:

Type:

RBS

Function:

Hardwire

Publication:

Mutalik,V2013

Description

Part of the Biofab collection, a huge assortment of experimentally validated constitutive promoters, RBSs and Terminators. RBSs in this collection were characterized in a bi-cistronic design (BCD), which employs an upstream RBS and coding region to the RBS of interest in order to insulate it from RNA secondary structure interference (from the 5'UTR region and the CDS N-terminal region). The design works by recruiting ribosomes to the upstream coding sequence, consequently unwinding any looped RNA and positioning the ribosome on top of the RBS of interest. The results were exceptionally robust.

Characterization

Parts were characterized on E. coli BW25113 in MOPS EZ Rich Medium at 37 °C in 96-well flow citometer assays. The authors then compared the expression of their genes of interest (fluorescent proteins) to the value predicted by the Salis Lab's RBS calculator to asses the robustness of their design and predictability of RBS sequences.

Sequences

RBS

TAAGGAGGT

Reference

Mutalik, V. K. et al. Precise and reliable gene expression via standard transcription and translation initiation elements. Nat. Methods 10, 354–360 (2013).

https://doi.org/10.1038/nmeth.2404

Extra References and External Links

Salis lab RBS calculator

Biofab collection